diff --git a/thread/TEST_MAPPING b/thread/TEST_MAPPING
index 34d67bb..40842f1 100644
--- a/thread/TEST_MAPPING
+++ b/thread/TEST_MAPPING
@@ -13,6 +13,9 @@
   "postsubmit": [
     {
       "name": "ThreadNetworkMultiDeviceTests"
+    },
+    {
+      "name": "ThreadNetworkTrelDisabledTests"
     }
   ]
 }
diff --git a/thread/tests/integration/Android.bp b/thread/tests/integration/Android.bp
index 8f082a4..798a51e 100644
--- a/thread/tests/integration/Android.bp
+++ b/thread/tests/integration/Android.bp
@@ -62,3 +62,23 @@
     ],
     compile_multilib: "both",
 }
+
+android_test {
+    name: "ThreadNetworkTrelDisabledTests",
+    platform_apis: true,
+    manifest: "AndroidManifest.xml",
+    test_config: "AndroidTestTrelDisabled.xml",
+    defaults: [
+        "framework-connectivity-test-defaults",
+        "ThreadNetworkIntegrationTestsDefaults",
+    ],
+    test_suites: [
+        "mts-tethering",
+        "general-tests",
+    ],
+    srcs: [
+        "src/**/*.java",
+        "src/**/*.kt",
+    ],
+    compile_multilib: "both",
+}
diff --git a/thread/tests/integration/AndroidTestTrelDisabled.xml b/thread/tests/integration/AndroidTestTrelDisabled.xml
new file mode 100644
index 0000000..600652a
--- /dev/null
+++ b/thread/tests/integration/AndroidTestTrelDisabled.xml
@@ -0,0 +1,57 @@
+<?xml version="1.0" encoding="utf-8"?>
+<!--
+    Copyright (C) 2024 The Android Open Source Project
+
+    Licensed under the Apache License, Version 2.0 (the "License");
+    you may not use this file except in compliance with the License.
+    You may obtain a copy of the License at
+
+        http://www.apache.org/licenses/LICENSE-2.0
+
+    Unless required by applicable law or agreed to in writing, software
+    distributed under the License is distributed on an "AS IS" BASIS,
+    WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+    See the License for the specific language governing permissions and
+    limitations under the License.
+ -->
+
+<configuration description="Config for Thread integration tests with TREL disabled">
+    <option name="test-tag" value="ThreadNetworkTrelDisabledTests" />
+    <option name="test-suite-tag" value="apct" />
+
+    <!--
+        Only run tests if the device under test is SDK version 34 (Android 14) or above.
+    -->
+    <object type="module_controller"
+            class="com.android.tradefed.testtype.suite.module.Sdk34ModuleController" />
+
+    <!-- Run tests in MTS only if the Tethering Mainline module is installed. -->
+    <object type="module_controller"
+            class="com.android.tradefed.testtype.suite.module.MainlineTestModuleController">
+        <option name="mainline-module-package-name" value="com.google.android.tethering" />
+    </object>
+
+    <object type="module_controller"
+            class="com.android.tradefed.testtype.suite.module.DeviceFeatureModuleController">
+        <option name="required-feature" value="android.hardware.thread_network" />
+    </object>
+
+    <target_preparer class="com.android.tradefed.targetprep.RootTargetPreparer" />
+
+    <!-- Install test -->
+    <target_preparer class="com.android.tradefed.targetprep.suite.SuiteApkInstaller">
+        <option name="test-file-name" value="ThreadNetworkTrelDisabledTests.apk" />
+        <option name="check-min-sdk" value="true" />
+        <option name="cleanup-apks" value="true" />
+    </target_preparer>
+
+    <test class="com.android.tradefed.testtype.AndroidJUnitTest" >
+        <option name="package" value="com.android.thread.tests.integration" />
+    </test>
+
+    <!-- Disable TREL for integration tests -->
+    <target_preparer class="com.android.tradefed.targetprep.FeatureFlagTargetPreparer">
+        <option name="flag-value"
+                value="thread_network/TrelFeature__enabled=false"/>
+    </target_preparer>
+</configuration>
diff --git a/thread/tests/integration/src/android/net/thread/ServiceDiscoveryTest.java b/thread/tests/integration/src/android/net/thread/ServiceDiscoveryTest.java
index 15259c8..6c2a9bb 100644
--- a/thread/tests/integration/src/android/net/thread/ServiceDiscoveryTest.java
+++ b/thread/tests/integration/src/android/net/thread/ServiceDiscoveryTest.java
@@ -30,6 +30,8 @@
 import static com.google.common.truth.Truth.assertThat;
 
 import static org.junit.Assert.assertThrows;
+import static org.junit.Assume.assumeFalse;
+import static org.junit.Assume.assumeTrue;
 
 import static java.nio.charset.StandardCharsets.UTF_8;
 import static java.util.concurrent.TimeUnit.MILLISECONDS;
@@ -50,6 +52,7 @@
 import androidx.test.filters.LargeTest;
 import androidx.test.runner.AndroidJUnit4;
 
+import com.android.net.module.util.DeviceConfigUtils;
 import com.android.net.module.util.HexDump;
 
 import com.google.common.truth.Correspondence;
@@ -445,9 +448,13 @@
     }
 
     @Test
-    // TODO: move this case out to BorderRoutingTest when the service discovery utilities
+    // TODO: move this case out of ServiceDiscoveryTest when the service discovery utilities
     // are decoupled from this test.
     public void trelFeatureFlagEnabled_trelServicePublished() throws Exception {
+        assumeTrue(
+                DeviceConfigUtils.getDeviceConfigPropertyBoolean(
+                        "thread_network", "TrelFeature__enabled", false));
+
         NsdServiceInfo discoveredService = discoverService(mNsdManager, "_trel._udp");
         assertThat(discoveredService).isNotNull();
         // Resolve service with the current TREL port, otherwise it may return stale service from
@@ -463,6 +470,17 @@
                 .isEqualTo(mOtCtl.getExtendedPanId().toLowerCase(Locale.ROOT));
     }
 
+    @Test
+    // TODO: move this case out of ServiceDiscoveryTest when the service discovery utilities
+    // are decoupled from this test.
+    public void trelFeatureFlagDisabled_trelServiceNotPublished() throws Exception {
+        assumeFalse(
+                DeviceConfigUtils.getDeviceConfigPropertyBoolean(
+                        "thread_network", "TrelFeature__enabled", false));
+
+        assertThrows(TimeoutException.class, () -> discoverService(mNsdManager, "_trel._udp"));
+    }
+
     private void registerService(NsdServiceInfo serviceInfo, RegistrationListener listener)
             throws InterruptedException, ExecutionException, TimeoutException {
         mNsdManager.registerService(serviceInfo, PROTOCOL_DNS_SD, listener);